A patient has been hospitalized into the infectious diseases department with complaints of recurrent episodes of diarrhea and vomiting, pain in the leg muscles, weakness, and dizziness. After the examination, the doctor provisionally diagnosed the patient with Cholera. What studies must be performed with the material obtained from the patient to make the express diagnosis?

• A. Serology
• B. Bacteriology
• C. Direct and indirect immunofluorescence
• D. Agglutination reaction
• E. Biological method